How can I attribute a specific shape to all miRNA in the ResNet Explore database?

Figure 1. miRNAs folder in Ariadne Ontology

Figure 2. New group containing all miRNAs

Figure 3. Option Choose Groups Used for Styling

Figure 4. Create a protein subcategory for miRNA

Figure 5. Attribute a specific shape to miRNAs

Figure 6. All miRNA are restyled

In ResNet Explore database, miRNA belongs to the protein entity category. By default, the miRNA shape is an ellipse, the generic protein shape in ResNet.

To attribute a specific shape to all miRNAs in the ResNet Explore database perform the following steps:

1. Browse the miRNAs folder in the Molecular function section of the Ariadne Ontology (Figure 1).

2. Create a new group and copy in the new group all the members of the miRNAs folder and sub-folders (Figure 2).

3. Save the new group and name it miRNA (Figure 2).

4. Create a new pathway and copy in the new pathway at least one miRNA.

5. Select a miRNA in the pathway and select Style > Save Style to Active Style Sheet As > Choose Groups Used for Styling (Figure 3).

6. In the pop-up window, click on the ‘Browse Groups’ button and select the group miRNA previously created (Figure 4). Click on OK.

7. Change the shape of the new protein subcategory called miRNA [i.e. Select the shape III-vertex] (Figure 5). Click OK.

8. Now all miRNAs in your database will be displayed with the new defined shape (style) and the new protein sub-category is reported in the palette tab (Figure 6).