DirectRegulation :


Owner	Public
URN	urn:agi-DirectRegulation:inout-urn:agi-llid:1616:out-urn:agi-llid:4217::positive
Entities	DAXX --+> MAP3K5
References	83
Effect	positive
Connectivity	2


Sentence	Expression of HSP27 also prevents the translocation of Daxx from the nucleus to the cytoplasm which is induced upon expression of Ask1 or stimulation of Fas.
Source	Curated
MedLine Reference	11193028:6


Sentence	Overexpression of catalase also inhibited relocalization of Daxx from the nucleus to the cytoplasm as well as association of Daxx with ASK1 during glucose deprivation.
CellLineName	DU 145
Source	Curated
Organ	prostate
MedLine Reference	14505347:2


Sentence	C, Daxx activates ASK1 .
CellLineName	293
Journal	J. Biol. Chem
Source	Curated
Journal Reference	v276 i42 p39103 (2001)
Journal Link	http://www.jbc.org/cgi/content/full/276/42/39103
Organism	plant
MedLine Reference	11495919:1081


Sentence	Unlike these endogenous inhibitors of ASK1 activity, TRAF2 and Daxx activate ASK1 kinase activity ( 36 , 37 ).
CellLineName	DU 145
Journal	J. Biol. Chem
Source	Curated
Journal Reference	v277 i48 p46566 (2002)
Journal Link	http://www.jbc.org/cgi/content/full/277/48/46566
MedLine Reference	12244106:1372


Sentence	Because it had been recently reported that Daxx activates ASK1 ( 12 ), the effect of mGSTM1-1 on Daxx-stimulated ASK1 activity was examined in the following experiments.
Journal	J. Biol. Chem
Source	Curated
Journal Reference	v276 i16 p12749 (2001)
Journal Link	http://www.jbc.org/cgi/content/full/276/16/12749
Organism	mouse
MedLine Reference	11278289:1154


Sentence	Daxx was originally identified as an interactor for the CD95 (Fas/APO-1) death receptor, and demonstrated to mediate activation of the MAPK JNK through activation of ASK1 (10 , 11) .
Journal	Cancer Res
Source	Curated
Journal Reference	v63 i23 p8271 (2003)
Journal Link	http://cancerres.aacrjournals.org/cgi/content/full/63/23/8271
MedLine Reference	14678985:1024


Sentence	Chang et al. ( 12 ) observed that Daxx activates ASK1 by displacing an inhibitory intramolecular interaction between the NH2 and COOH termini of the kinase, thereby opening up the kinase into an active conformation.
Journal	J. Biol. Chem
Source	Curated
Journal Reference	v278 i47 p47245 (2003)
Journal Link	http://www.jbc.org/cgi/content/full/278/47/47245
Organism	mouse
MedLine Reference	12968034:1035


Sentence	Still, a role for Daxx in signaling to ASK1 and the SAPKs cannot be ruled out at this time; some investigators reliably observe that Daxx can activate both ASK1 and the SAPKs ( 4 , 40 ), while others do not observe SAPK activation upon overexpression of Daxx ( 36 ).
Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v20 i6 p2198 (2000)
Journal Link	http://mcb.asm.org/cgi/content/full/20/6/2198
MedLine Reference	10688666:1042


Sentence	In particular, a major problem has been the finding that Daxx is a nuclear protein, a result which appeared inconsistent with the demonstration that upon Fas stimulation Daxx mediates the formation of a multiprotein complex made of Fas, Ask1, and Daxx ( 7 , 8 , 66 ).
Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v20 i20 p7602 (2000)
Journal Link	http://mcb.asm.org/cgi/content/full/20/20/7602
CellType	fibroblast
MedLine Reference	11003656:1292


Journal	J. Exp. Med
Source	Curated
Journal Reference	v191 i4 p631 (2000)
Journal Link	http://www.jem.org/cgi/content/full/191/4/631


Journal	J. Biol. Chem
Source	Curated
Journal Reference	v276 i8 p6030 (2001)
Journal Link	http://www.jbc.org/cgi/content/full/276/8/6030
MedLine Reference	11096076:1212


Journal	PNAS
Source	Curated
Journal Reference	v96 i4 p1252 (1999)
Journal Link	http://www.pnas.org/cgi/content/full/96/4/1252
MedLine Reference	9990010:1116


Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v20 i5 p1784 (2000)
Journal Link	http://mcb.asm.org/cgi/content/full/20/5/1784
Organism	mouse
MedLine Reference	10669753:1043


Journal	J. Exp. Med
Source	Curated
Journal Reference	v191 i4 p631 (2000)
Journal Link	http://www.jem.org/cgi/content/full/191/4/631
Organism	mouse
MedLine Reference	10684855:1230


CellLineName	293
Journal	Science
Source	Curated
Journal Reference	v281 i5384 p1860 (1998)
Journal Link	http://www.sciencemag.org/cgi/content/full/281/5384/1860
MedLine Reference	9743501:1035


Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v20 i20 p7602 (2000)
Journal Link	http://mcb.asm.org/cgi/content/full/20/20/7602
Organism	mouse
CellType	fibroblast
MedLine Reference	11003655:1292


CellLineName	293
Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v23 i1 p322 (2003)
Journal Link	http://mcb.asm.org/cgi/content/full/23/1/322
MedLine Reference	12482984:1213


Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v22 i22 p7721 (2002)
Journal Link	http://mcb.asm.org/cgi/content/full/22/22/7721
Organism	mouse
MedLine Reference	12391142:1248


Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v20 i6 p2198 (2000)
Journal Link	http://mcb.asm.org/cgi/content/full/20/6/2198
Organism	mouse
MedLine Reference	10688665:1042


Journal	J. Immunol
Source	Curated
Journal Reference	v165 i3 p1337 (2000)
Journal Link	http://www.jimmunol.org/cgi/content/full/165/3/1337
Organism	mouse
CellType	fibroblast
MedLine Reference	10903735:1024


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


CellLineName	NIH 3T3
Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


CellLineName	NIH 3T3
Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v24 i15 p6763 (2004)
Journal Link	http://mcb.asm.org/cgi/content/full/24/15/6763
Organism	Rattus norvegicus
MedLine Reference	15254243:1211


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Exp. Med
Source	Curated
Journal Reference	v191 i4 p631 (2000)
Journal Link	http://www.jem.org/cgi/content/full/191/4/631


CellLineName	NIH 3T3
Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


CellLineName	NIH 3T3
Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Biol. Chem
Source	Curated
Journal Reference	v276 i39 p36071 (2001)
Journal Link	http://www.jbc.org/cgi/content/full/276/39/36071
MedLine Reference	11493600:1129


Source	Curated
MedLine Reference	12858332:3


Journal	J. Biol. Chem
Source	Curated
Journal Reference	v279 i19 p20369 (2004)
Journal Link	http://www.jbc.org/cgi/content/full/279/19/20369
MedLine Reference	14990586:1023


Journal	J. Biol. Chem
Source	Curated
Journal Reference	v279 i45 p46595 (2004)
Journal Link	http://www.jbc.org/cgi/content/full/279/45/46595
MedLine Reference	15328343:1305


Journal	Cell Sci
Source	Curated
Journal Reference	v117 i13 p2641 (2004)
Journal Link	http://jcs.biologists.org/cgi/content/full/117/13/2641
MedLine Reference	15169835:1159


Sentence	Tumor necrosis factor activation of ASK1 is mediated by the adaptor protein TRAF2, and Fas activation of ASK1 is mediated by the Daxx adaptor (Chang et al., 1998; Nishitoh et al., 1998 ).
Journal	Cell
Source	Curated
Journal Reference	v105 i2 p221 (2001)
Journal Link	http://www.cell.com/cgi/content/full/105/2/221
MedLine Reference	3297069081


Sentence	Fas also activates a caspase-independent pathway which correlates with Fas-induced translocation of Daxx from the nucleus to the cytoplasm and involves the interaction of Daxx with Fas and Ask1.
Source	Curated
MedLine Reference	11193028:4


Sentence	The Daxx-induced ASK1 activation leads to apoptotic cell death through the activation of the JNK/SAPK signaling pathway ( 12 , 27 ).
Journal	J. Biol. Chem
Source	Curated
Journal Reference	v276 i16 p12749 (2001)
Journal Link	http://www.jbc.org/cgi/content/full/276/16/12749
Organism	Mus musculus
MedLine Reference	11278289:1046


Sentence	The strong Daxx-interacting 175RH and 273RH mutants strongly inhibited the activation of ASK1 induced by Daxx (Fig. 5 , lanes 5 and 6).
CellLineName	293
Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v23 i1 p322 (2003)
Journal Link	http://mcb.asm.org/cgi/content/full/23/1/322
MedLine Reference	12482984:1215


Sentence	Daxx directly interacts with ASK1 ( 4 ), a mitogen-activated protein kinase kinase kinase ( 24 , 53 , 63 ), which in turn activates the JNK- and p38-dependent cascades.
Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v23 i1 p322 (2003)
Journal Link	http://mcb.asm.org/cgi/content/full/23/1/322
MedLine Reference	12482984:1050


Sentence	Chang et al. ( 12 ) observed that Daxx activates ASK1 by displacing an inhibitory intramolecular interaction between the NH2 and COOH termini of the kinase, thereby opening up the kinase into an active conformation.
Journal	J. Biol. Chem
Source	Curated
Journal Reference	v278 i47 p47245 (2003)
Journal Link	http://www.jbc.org/cgi/content/full/278/47/47245
Organism	Mus musculus
MedLine Reference	12968034:1034


Sentence	After Fas ligation, Fas recruits an adaptor protein called Daxx that interacts with apoptosis signal-regulating kinase 1 (ASK1 ) activating JNK/SAPK and p38 MAPK by phosphorylation ( 8 , 9 ).
Journal	J. Biol. Chem
Source	Curated
Journal Reference	v276 i42 p39103 (2001)
Journal Link	http://www.jbc.org/cgi/content/full/276/42/39103
MedLine Reference	11495919:1024


Sentence	TRAF2 and Daxx have been shown to mediate ASK1 activation by TNF- and Fas, respectively ( 4 , 26 ), whereas thioredoxin, p21, 14-3-3, and GST µ function as cellular inhibitors of ASK1 ( 1 , 6 , 30 , 36 ).
Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v22 i22 p7721 (2002)
Journal Link	http://mcb.asm.org/cgi/content/full/22/22/7721
Organism	Mus musculus
MedLine Reference	12391142:1249


Sentence	The interaction between Daxx and Ask1 was confirmed by showing that Ask1 from extracts of 293 cells was retained on immobilized GST-55.1 (Fig. 3 C, lane 2) or coimmunoprecipitated with antibodies against Daxx (data not shown).
CellLineName	293
Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v20 i20 p7602 (2000)
Journal Link	http://mcb.asm.org/cgi/content/full/20/20/7602
MedLine Reference	11003656:1169


Sentence	The similarity of the mechanisms involved in the activation of the two functions of Ask1 makes it plausible that in some cellular context the interaction of Daxx with Ask1 results in the activation of the kinase activity rather than in the cell-death activity linked to the N terminus.
Journal	J. Biol. Chem
Source	Curated
Journal Reference	v276 i39 p36071 (2001)
Journal Link	http://www.jbc.org/cgi/content/full/276/39/36071
MedLine Reference	11493600:1132


Sentence	In particular, a major problem has been the finding that Daxx is a nuclear protein, a result which appeared inconsistent with the demonstration that upon Fas stimulation Daxx mediates the formation of a multiprotein complex made of Fas, Ask1, and Daxx ( 7 , 8 , 66 ).
Journal	Mol. Cell. Biol
Source	Curated
Journal Reference	v20 i20 p7602 (2000)
Journal Link	http://mcb.asm.org/cgi/content/full/20/20/7602
MedLine Reference	11003656:1289


Sentence	RESULTS TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES RESULTS DISCUSSION REFERENCES Oligomerization of ASK1 and Interaction between Daxx and ASK1 during Glucose Deprivation ?Previous studies have shown that oligomerization of ASK1 occurs during treatment with TNF in human embryonic kidney 293 cells ( 11 ).
CellLineName	293
Journal	J. Biol. Chem
Source	Curated
Journal Reference	v278 i47 p47245 (2003)
Journal Link	http://www.jbc.org/cgi/content/full/278/47/47245
Organism	Mus musculus
Organ	kidney
MedLine Reference	12968034:1098


Sentence	JNK1 was immunoprecipitated with anti-FLAG, and in vitro kinase assay with 1 µg of GST-cJun(1-79) was performed as described ( 4 ). (Top) Phosphorylation of GST-cJun(1-79). (Bottom) Immunoblotting of immunoprecipitated FLAG-JNK1. [View Larger Versions of these Images (15 + 18K GIF file)] Because MAP3Ks such as Raf directly interact with upstream signaling proteins ( 5 ), we assayed physical interaction between Daxx and ASK1 by coimmunoprecipitation from transfected 293T cells.
CellLineName	293T
Journal	Science
Source	Curated
Journal Reference	v281 i5384 p1860 (1998)
Journal Link	http://www.sciencemag.org/cgi/content/full/281/5384/1860
MedLine Reference	9743501:1065


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Exp. Med
Source	Curated
Journal Reference	v191 i4 p631 (2000)
Journal Link	http://www.jem.org/cgi/content/full/191/4/631


CellLineName	NIH 3T3
Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


CellLineName	NIH 3T3
Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Journal	J. Exp. Med
Source	Curated
Journal Reference	v191 i4 p631 (2000)
Journal Link	http://www.jem.org/cgi/content/full/191/4/631


CellLineName	NIH 3T3
Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


CellLineName	NIH 3T3
Journal	J. Cell Biol
Source	Curated
Journal Reference	v163 i1 p71 (2003)
Journal Link	http://www.jcb.org/cgi/content/full/163/1/71


Sentence	Tumor necrosis factor activation of ASK1 is mediated by the adaptor protein TRAF2, and Fas activation of ASK1 is mediated by the Daxx adaptor (Chang et al., 1998; Nishitoh et al., 1998 ).
Journal	Cell
Source	Curated
Journal Reference	v105 i2 p221 (2001)
Journal Link	http://www.cell.com/cgi/content/full/105/2/221
MedLine Reference	3640586377